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bicinchonic acid protein assay kit  (Thermo Fisher)


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    Structured Review

    Thermo Fisher bicinchonic acid protein assay kit
    Bicinchonic Acid Protein Assay Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bicinchonic+acid+assay+kit/BCA+Protein+Assay+Kit/pmc12892636-84-9-14
    Average 99 stars, based on 1 article reviews
    bicinchonic acid protein assay kit - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Acid Assay:

    Article Title: Pharmacogenomics of poor drug metabolism in greyhounds: Canine P450 oxidoreductase genetic variation, breed heterogeneity, and functional characterization
    Article Snippet: .. Microsomal protein concentrations were measured using the Pierce bicinchonic acid assay kit (Thermo Fisher Scientific). ..

    Article Title: Reversible assembly and disassembly of V-ATPase during the lysosome regeneration cycle
    Article Snippet: Isolation of the lysosomes was then as previously described (Rofe and Pryor, 2016), but with final elution from the MiniMACS Large Cell Column (Miltenyi BioTec, Bisley, UK) into 150 μL STM. .. Protein concentration of subcellular fractions was measured with a Pierce bicinchonic acid assay kit (Thermo Fisher Scientific) using bovine serum albumin (BSA) to generate a standard curve. .. Immunoblotting was as previously described (Davis et al., 2021) except that SDS–PAGE was carried out using Millipore mPAGE 10% Bis-Tris Precast Gels (MP10W10/ MP10W12/ MP10W15) with MES Volume 35 May 1, 2024 Lysosomal V-ATPase assembly/disassembly | 13 SDS running buffer (MPMES) from Merck, before protein transfer to PVDF membranes.

    Article Title: Withanolide-A treatment exerts a neuroprotective effect via inhibiting neuroinflammation in the hippocampus after pilocarpine-induced status epilepticus.
    Article Snippet: Status epilepticus (SE) is a medical emergency with high mortality and a risk factor for the development of chronic epilepsy.. Given that effective treatments for the pathophysiology following SE are still lacking, suppressing pathophysiological mechanisms of SE may be important to inhibit epileptogenesis.. Withanolide-A (WA), a major bioactive component of Withania somnifera, is a potential medicinal natural compound showing improvement of some neurological diseases, such as cerebral ischemia.

    Article Title: Identification and evaluation of fructose-bisphosphate aldolase B as a potential diagnostic biomarker in choledochal cysts patients: a quantitative proteomic analysis
    Article Snippet: .. High-abundance proteins were removed with Pierce Top 12 (cat 85165, Thermo Scientific, Rockform, IL, USA) and the total protein concentration was measured using the bicinchonic acid assay kit (cat 23227, Pierce, Thermo Scientific, Rockform, IL, USA). .. Total protein (350 µg) was dried in a high-speed vacuum (Savant, Thermofisher) and dissolved in 6 M urea and 100 mM Tris-HCl (pH 7.4).

    Article Title: Pharmacogenomics of poor drug metabolism in greyhounds: Canine P450 oxidoreductase genetic variation, breed heterogeneity, and functional characterization.
    Article Snippet: .. Microsomal protein concentrations were measured using the Pierce bicinchonic acid assay kit (Thermo Fisher Scientific). ..

    Article Title:
    Article Snippet: His tagged ExoA was purified by the addition of an elution buffer according to manufacturer's instructions (QIA expressionist, QIAGEN) containing 250 mM imidazole with the addition of 20% w/v glycerol and 5% w/v glucose. .. Protein yields were estimated using a bicinchonic acid assay kit according to manufacturer's instructions (Pierce® Biotechnology BCA protein Assay Kit, U.S.A.). .. For large-scale protein purification, material was isolated using GE Healthcare HIS trap columns and an AKTA purifier with an imidazole gradient of 30 mM to 500 mM.

    Protein Concentration:

    Article Title: Reversible assembly and disassembly of V-ATPase during the lysosome regeneration cycle
    Article Snippet: Isolation of the lysosomes was then as previously described (Rofe and Pryor, 2016), but with final elution from the MiniMACS Large Cell Column (Miltenyi BioTec, Bisley, UK) into 150 μL STM. .. Protein concentration of subcellular fractions was measured with a Pierce bicinchonic acid assay kit (Thermo Fisher Scientific) using bovine serum albumin (BSA) to generate a standard curve. .. Immunoblotting was as previously described (Davis et al., 2021) except that SDS–PAGE was carried out using Millipore mPAGE 10% Bis-Tris Precast Gels (MP10W10/ MP10W12/ MP10W15) with MES Volume 35 May 1, 2024 Lysosomal V-ATPase assembly/disassembly | 13 SDS running buffer (MPMES) from Merck, before protein transfer to PVDF membranes.

    Article Title: Withanolide-A treatment exerts a neuroprotective effect via inhibiting neuroinflammation in the hippocampus after pilocarpine-induced status epilepticus.
    Article Snippet: Status epilepticus (SE) is a medical emergency with high mortality and a risk factor for the development of chronic epilepsy.. Given that effective treatments for the pathophysiology following SE are still lacking, suppressing pathophysiological mechanisms of SE may be important to inhibit epileptogenesis.. Withanolide-A (WA), a major bioactive component of Withania somnifera, is a potential medicinal natural compound showing improvement of some neurological diseases, such as cerebral ischemia.

    Article Title: Identification and evaluation of fructose-bisphosphate aldolase B as a potential diagnostic biomarker in choledochal cysts patients: a quantitative proteomic analysis
    Article Snippet: .. High-abundance proteins were removed with Pierce Top 12 (cat 85165, Thermo Scientific, Rockform, IL, USA) and the total protein concentration was measured using the bicinchonic acid assay kit (cat 23227, Pierce, Thermo Scientific, Rockform, IL, USA). .. Total protein (350 µg) was dried in a high-speed vacuum (Savant, Thermofisher) and dissolved in 6 M urea and 100 mM Tris-HCl (pH 7.4).

    Mouse Assay:

    Article Title: Withanolide-A treatment exerts a neuroprotective effect via inhibiting neuroinflammation in the hippocampus after pilocarpine-induced status epilepticus.
    Article Snippet: Status epilepticus (SE) is a medical emergency with high mortality and a risk factor for the development of chronic epilepsy.. Given that effective treatments for the pathophysiology following SE are still lacking, suppressing pathophysiological mechanisms of SE may be important to inhibit epileptogenesis.. Withanolide-A (WA), a major bioactive component of Withania somnifera, is a potential medicinal natural compound showing improvement of some neurological diseases, such as cerebral ischemia.

    Bicinchoninic Acid Protein Assay:

    Article Title:
    Article Snippet: His tagged ExoA was purified by the addition of an elution buffer according to manufacturer's instructions (QIA expressionist, QIAGEN) containing 250 mM imidazole with the addition of 20% w/v glycerol and 5% w/v glucose. .. Protein yields were estimated using a bicinchonic acid assay kit according to manufacturer's instructions (Pierce® Biotechnology BCA protein Assay Kit, U.S.A.). .. For large-scale protein purification, material was isolated using GE Healthcare HIS trap columns and an AKTA purifier with an imidazole gradient of 30 mM to 500 mM.



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    Thermo Fisher bicinchonic acid bca assay
    A) Schematic figure showing the collection of the whole apical wash from HAE cultures. B) Viscosity of the apical wash samples from each treatment group quantified using a microviscometer. C) The total protein in the apical wash was quantified using a <t>bicinchonic</t> acid based colorimetric reaction. The concentration of total protein in each sample was determined using a standard curve of bovine serum albumin and D) the percent change in the absorbance values was calculated in relation to the untreated group. E) The DNA in the apical wash quantified using 3,5-diaminbenzoic acid (DABA) to produce a fluorescent reaction. The percent change in the fluorescence was calculated compared to the untreated group. Statistical significance in B, C, D and E was determined using one-way ANOVAs with Tukey’s multiple comparison tests (ns, no significance, * p < 0.05, ** p < 0.01, **** p < 0.0001).
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    Image Search Results


    A) Schematic figure showing the collection of the whole apical wash from HAE cultures. B) Viscosity of the apical wash samples from each treatment group quantified using a microviscometer. C) The total protein in the apical wash was quantified using a bicinchonic acid based colorimetric reaction. The concentration of total protein in each sample was determined using a standard curve of bovine serum albumin and D) the percent change in the absorbance values was calculated in relation to the untreated group. E) The DNA in the apical wash quantified using 3,5-diaminbenzoic acid (DABA) to produce a fluorescent reaction. The percent change in the fluorescence was calculated compared to the untreated group. Statistical significance in B, C, D and E was determined using one-way ANOVAs with Tukey’s multiple comparison tests (ns, no significance, * p < 0.05, ** p < 0.01, **** p < 0.0001).

    Journal: bioRxiv

    Article Title: Myeloperoxidase impairs mucociliary transport on human airway epithelium

    doi: 10.1101/2025.11.17.688903

    Figure Lengend Snippet: A) Schematic figure showing the collection of the whole apical wash from HAE cultures. B) Viscosity of the apical wash samples from each treatment group quantified using a microviscometer. C) The total protein in the apical wash was quantified using a bicinchonic acid based colorimetric reaction. The concentration of total protein in each sample was determined using a standard curve of bovine serum albumin and D) the percent change in the absorbance values was calculated in relation to the untreated group. E) The DNA in the apical wash quantified using 3,5-diaminbenzoic acid (DABA) to produce a fluorescent reaction. The percent change in the fluorescence was calculated compared to the untreated group. Statistical significance in B, C, D and E was determined using one-way ANOVAs with Tukey’s multiple comparison tests (ns, no significance, * p < 0.05, ** p < 0.01, **** p < 0.0001).

    Article Snippet: The total protein in the apical wash of HAE cells was determined using a bicinchonic acid (BCA) assay (Thermo Fisher Scientific), according to kit instructions.

    Techniques: Viscosity, Concentration Assay, Fluorescence, Comparison